A refined genome phage display methodology delineates the human antibody response in patients with Chagas disease

A refined genome phage display methodology delineates the human antibody response in patients with Chagas disease

Blog Article

Summary: Large-scale mapping of antigens and epitopes is pivotal for developing Used Roller Blade immunotherapies but challenging, especially for eukaryotic pathogens, owing to their large genomes.Here, we developed an integrated platform for genome phage display (gPhage) to show that unbiased libraries of the eukaryotic parasite Trypanosoma cruzi enable the identification of thousands of antigens recognized by serum samples from patients with Chagas disease.Because most of these antigens are hypothetical proteins, gPhage provides evidence of their expression during infection.

We built and validated a comprehensive map of Chagas disease antibody response to show how linear and putative conformation epitopes, many rich in repetitive elements, allow the parasite to evade a buildup of neutralizing antibodies directed against FERM PROTEINS VANILLA protein domains that mediate infection pathogenesis.Thus, the gPhage platform is a reproducible and effective tool for rapid simultaneous identification of epitopes and antigens, not only in Chagas disease but perhaps also in globally emerging/reemerging acute pathogens.